Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein
Identifieur interne : 006176 ( Main/Exploration ); précédent : 006175; suivant : 006177Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein
Auteurs : Valérie Campanacci ; Marie-Pierre Egloff ; Sonia Longhi ; François Ferron ; Corinne Rancurel ; Aurelia Salomoni ; Cécile Durousseau ; Fabienne Tocque ; Nicolas Brémond ; Jessika C. Dobbe ; Eric J. Snijder ; Bruno Canard ; Christian CambillauSource :
- Acta Crystallographica Section D [ 1399-0047 ] ; 2003-09.
Descripteurs français
- KwdFr :
- Alignement de séquences, Clonage moléculaire, Cristallisation, Cristallographie aux rayons X, Génomique, Protéines de liaison à l'ARN (), Protéines virales (), Protéines virales non structurales (), Protéines virales non structurales (génétique), Protéines virales non structurales (isolement et purification), Séquence d'acides aminés, Virus du SRAS (), Virus du SRAS (génétique).
- MESH :
- génétique : Protéines virales non structurales, Virus du SRAS.
- isolement et purification : Protéines virales non structurales.
- Alignement de séquences, Clonage moléculaire, Cristallisation, Cristallographie aux rayons X, Génomique, Protéines de liaison à l'ARN, Protéines virales, Protéines virales non structurales, Séquence d'acides aminés, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Sequence, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Genomics, RNA-Binding Proteins (chemistry), SARS Virus (chemistry), SARS Virus (genetics), Sequence Alignment, Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (isolation & purification), Viral Proteins (chemistry).
- MESH :
- chemical , chemistry : RNA-Binding Proteins, Viral Nonstructural Proteins, Viral Proteins.
- chemistry : SARS Virus.
- genetics : SARS Virus, Viral Nonstructural Proteins.
- chemical , isolation & purification : Viral Nonstructural Proteins.
- Amino Acid Sequence, Cloning, Molecular, Crystallization, Crystallography, X-Ray, Genomics, Sequence Alignment.
Abstract
The aetiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive‐stranded RNA virus (SARS‐CoV) belonging to the Coronaviridae family and its genome differs substantially from those of other known coronaviruses. SARS‐CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS‐CoV whole‐genome approach has been developed aimed at determining the crystal structure of all of its proteins or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20–30 mature proteins. The functions of many of these polypeptides, including the Nsp9–Nsp10 replicase‐cleavage products, are still unknown. Here, the cloning, Escherichia coli expression, purification and crystallization of the SARS‐CoV Nsp9 protein, the first SARS‐CoV protein to be crystallized, are reported. Nsp9 crystals diffract to 2.8 Å resolution and belong to space group P61/522, with unit‐cell parameters a = b = 89.7, c = 136.7 Å. With two molecules in the asymmetric unit, the solvent content is 60% (VM = 3.1 Å3 Da−1).
Url:
- https://api.istex.fr/ark:/67375/WNG-VJ7RMB0T-N/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161644
DOI: 10.1107/S0907444903016779
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000230
- to stream Istex, to step Curation: 000230
- to stream Istex, to step Checkpoint: 002179
- to stream Pmc, to step Corpus: 000B47
- to stream Pmc, to step Curation: 000B47
- to stream Pmc, to step Checkpoint: 001790
- to stream PubMed, to step Corpus: 003221
- to stream PubMed, to step Curation: 003221
- to stream PubMed, to step Checkpoint: 003044
- to stream Ncbi, to step Merge: 000294
- to stream Ncbi, to step Curation: 000294
- to stream Ncbi, to step Checkpoint: 000294
- to stream Main, to step Merge: 006681
- to stream Main, to step Curation: 006176
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein</title><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name></author><author><name sortKey="Egloff, Marie Ierre" sort="Egloff, Marie Ierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name></author><author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name></author><author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name></author><author><name sortKey="Salomoni, Aurelia" sort="Salomoni, Aurelia" uniqKey="Salomoni A" first="Aurelia" last="Salomoni">Aurelia Salomoni</name></author><author><name sortKey="Durousseau, Cecile" sort="Durousseau, Cecile" uniqKey="Durousseau C" first="Cécile" last="Durousseau">Cécile Durousseau</name></author><author><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></author><author><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name></author><author><name sortKey="Dobbe, Jessika C" sort="Dobbe, Jessika C" uniqKey="Dobbe J" first="Jessika C." last="Dobbe">Jessika C. Dobbe</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AC67EE175018885E531826A5374344F32BDF6060</idno><date when="2003" year="2003">2003</date><idno type="doi">10.1107/S0907444903016779</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-VJ7RMB0T-N/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000230</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000230</idno><idno type="wicri:Area/Istex/Curation">000230</idno><idno type="wicri:Area/Istex/Checkpoint">002179</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">002179</idno><idno type="wicri:doubleKey">1399-0047:2003:Campanacci V:structural:genomics:of</idno><idno type="wicri:source">PMC</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161644</idno><idno type="RBID">PMC:7161644</idno><idno type="wicri:Area/Pmc/Corpus">000B47</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B47</idno><idno type="wicri:Area/Pmc/Curation">000B47</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B47</idno><idno type="wicri:Area/Pmc/Checkpoint">001790</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001790</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:12925794</idno><idno type="wicri:Area/PubMed/Corpus">003221</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003221</idno><idno type="wicri:Area/PubMed/Curation">003221</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003221</idno><idno type="wicri:Area/PubMed/Checkpoint">003044</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003044</idno><idno type="wicri:Area/Ncbi/Merge">000294</idno><idno type="wicri:Area/Ncbi/Curation">000294</idno><idno type="wicri:Area/Ncbi/Checkpoint">000294</idno><idno type="wicri:doubleKey">0907-4449:2003:Campanacci V:structural:genomics:of</idno><idno type="wicri:Area/Main/Merge">006681</idno><idno type="wicri:Area/Main/Curation">006176</idno><idno type="wicri:Area/Main/Exploration">006176</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein</title><author><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name></author><author><name sortKey="Egloff, Marie Ierre" sort="Egloff, Marie Ierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name></author><author><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name></author><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name></author><author><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name></author><author><name sortKey="Salomoni, Aurelia" sort="Salomoni, Aurelia" uniqKey="Salomoni A" first="Aurelia" last="Salomoni">Aurelia Salomoni</name></author><author><name sortKey="Durousseau, Cecile" sort="Durousseau, Cecile" uniqKey="Durousseau C" first="Cécile" last="Durousseau">Cécile Durousseau</name></author><author><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></author><author><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name></author><author><name sortKey="Dobbe, Jessika C" sort="Dobbe, Jessika C" uniqKey="Dobbe J" first="Jessika C." last="Dobbe">Jessika C. Dobbe</name></author><author><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name></author><author><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name></author></analytic><monogr></monogr><series><title level="j" type="main">Acta Crystallographica Section D</title><title level="j" type="alt">ACTA CRYSTALLOGRAPHICA D</title><idno type="ISSN">1399-0047</idno><idno type="eISSN">1399-0047</idno><imprint><biblScope unit="vol">59</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="1628">1628</biblScope><biblScope unit="page" to="1631">1631</biblScope><publisher>Munksgaard International Publishers</publisher><pubPlace>5 Abbey Square, Chester, Cheshire CH1 2HU, England</pubPlace><date type="published" when="2003-09">2003-09</date></imprint><idno type="ISSN">1399-0047</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1399-0047</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Genomics</term><term>RNA-Binding Proteins (chemistry)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>Sequence Alignment</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (isolation & purification)</term><term>Viral Proteins (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences</term><term>Clonage moléculaire</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Génomique</term><term>Protéines de liaison à l'ARN ()</term><term>Protéines virales ()</term><term>Protéines virales non structurales ()</term><term>Protéines virales non structurales (génétique)</term><term>Protéines virales non structurales (isolement et purification)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA-Binding Proteins</term><term>Viral Nonstructural Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales non structurales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines virales non structurales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Crystallization</term><term>Crystallography, X-Ray</term><term>Genomics</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Clonage moléculaire</term><term>Cristallisation</term><term>Cristallographie aux rayons X</term><term>Génomique</term><term>Protéines de liaison à l'ARN</term><term>Protéines virales</term><term>Protéines virales non structurales</term><term>Séquence d'acides aminés</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aetiologic agent of the recent epidemics of Severe Acute Respiratory Syndrome (SARS) is a positive‐stranded RNA virus (SARS‐CoV) belonging to the Coronaviridae family and its genome differs substantially from those of other known coronaviruses. SARS‐CoV is transmissible mainly by the respiratory route and to date there is no vaccine and no prophylactic or therapeutic treatments against this agent. A SARS‐CoV whole‐genome approach has been developed aimed at determining the crystal structure of all of its proteins or domains. These studies are expected to greatly facilitate drug design. The genomes of coronaviruses are between 27 and 31.5 kbp in length, the largest of the known RNA viruses, and encode 20–30 mature proteins. The functions of many of these polypeptides, including the Nsp9–Nsp10 replicase‐cleavage products, are still unknown. Here, the cloning, Escherichia coli expression, purification and crystallization of the SARS‐CoV Nsp9 protein, the first SARS‐CoV protein to be crystallized, are reported. Nsp9 crystals diffract to 2.8 Å resolution and belong to space group P61/522, with unit‐cell parameters a = b = 89.7, c = 136.7 Å. With two molecules in the asymmetric unit, the solvent content is 60% (VM = 3.1 Å3 Da−1).</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Bremond, Nicolas" sort="Bremond, Nicolas" uniqKey="Bremond N" first="Nicolas" last="Brémond">Nicolas Brémond</name><name sortKey="Cambillau, Christian" sort="Cambillau, Christian" uniqKey="Cambillau C" first="Christian" last="Cambillau">Christian Cambillau</name><name sortKey="Campanacci, Valerie" sort="Campanacci, Valerie" uniqKey="Campanacci V" first="Valérie" last="Campanacci">Valérie Campanacci</name><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><name sortKey="Dobbe, Jessika C" sort="Dobbe, Jessika C" uniqKey="Dobbe J" first="Jessika C." last="Dobbe">Jessika C. Dobbe</name><name sortKey="Durousseau, Cecile" sort="Durousseau, Cecile" uniqKey="Durousseau C" first="Cécile" last="Durousseau">Cécile Durousseau</name><name sortKey="Egloff, Marie Ierre" sort="Egloff, Marie Ierre" uniqKey="Egloff M" first="Marie-Pierre" last="Egloff">Marie-Pierre Egloff</name><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><name sortKey="Longhi, Sonia" sort="Longhi, Sonia" uniqKey="Longhi S" first="Sonia" last="Longhi">Sonia Longhi</name><name sortKey="Rancurel, Corinne" sort="Rancurel, Corinne" uniqKey="Rancurel C" first="Corinne" last="Rancurel">Corinne Rancurel</name><name sortKey="Salomoni, Aurelia" sort="Salomoni, Aurelia" uniqKey="Salomoni A" first="Aurelia" last="Salomoni">Aurelia Salomoni</name><name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name><name sortKey="Tocque, Fabienne" sort="Tocque, Fabienne" uniqKey="Tocque F" first="Fabienne" last="Tocque">Fabienne Tocque</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006176 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006176 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:AC67EE175018885E531826A5374344F32BDF6060
|texte= Structural genomics of the SARS coronavirus: cloning, expression, crystallization and preliminary crystallographic study of the Nsp9 protein
}}
| This area was generated with Dilib version V0.6.33. |  |